E-GEOD-40345 - Human Mesothelioma MicroRNA Study Part 1

Status
Released on 1 October 2013, last updated on 2 June 2014
Organism
Homo sapiens
Samples (31)
Array (1)
Protocols (5)
Description
MicroRNA expression profiles of malignant pleural mesothelioma (MPM) specimens were analyzed to identify novel microRNA that are potentially involved in the oncogenic transformation of human pleural cells. In addition to several novel MPM-associated microRNAs, we observed that the expression level of microRNA-1 was significantly lower in tumors as compared to normal pleural specimens. Subsequently, pre-mir of microRNA-1 was introduced into MPM cell lines to overexpress this microRNA. Phenotypic changes of these altered cells were assayed. The cellular proliferation rate was significantly inhibited after overexpression of microRNA-1. Early and late apoptosis were measured by Annexin V and TUNEL assays, respectively. Taken together, these data suggested that overexpression of microRNA-1 induced apoptosis in these MPM cell lines, acting as a tumor suppressor. We confirmed our observations by assessing in the transduced MPM cells cell cycle-related genes, pro-apoptotic and anti-apoptotic genes, which all showed coordinated, significant changes characteristic of the apoptotic phenotype. Thus, further investigation and validation of our microRNA database of MPM may elucidate previously unrecognized molecular pathways and/ or mechanisms by identifying novel microRNAs that are involved in malignant transformation. Our study has now found microRNA-1 to be one of these MPM-associated microRNAs, with potential pathogenic and therapeutic significance. MicroRNA microarray transcriptional profiling studies of 25 MPM primary tumors as well 6 normal pleural samples from an unmatched patient cohort as normal comparators were performed.
Experiment type
transcription profiling by array 
Contacts
Ming Zheng <mzheng@stanford.edu>, Chuong D Hoang, Yue Xu
Citation
microRNA-1 Induces Growth Arrest and Apoptosis in Malignant Mesothelioma. Xu Y, Zheng M, Merritt RE, Shrager JB, Wakelee H, Kratzke RA, Hoang CD. , Europe PMC 23828229
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-40345.idf.txt
Sample and data relationshipE-GEOD-40345.sdrf.txt
Processed data (1)E-GEOD-40345.processed.1.zip
Array designA-GEOD-8179.adf.txt
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