E-GEOD-40282 - Gene expression data of Lsd1fl/fl and Lsd1fl/fl Mx1Cre Gr1dim Mac1 granulocytic progenitor cells.

Status
Released on 25 June 2013, last updated on 2 June 2014
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (7)
Description
We discovered that mice with hematopoietic-specific deletion of Lsd1 lacked Gr-1+ Mac1+ neutrophilic granulocytes whereas the numbers of Gr-1dim Mac1+ granulocytic progenitor cells was increased. To determine the genes altered by Lsd1-loss, Gr-1dim Mac1+ granulocytic progenitor cells from Lsd1fl/fl and Lsd1fl/fl Mx1Cre mice were FACS-purified to be analyzed by gene expression profiling. Primary Gr-1dim Mac1+ granulocytic progenitor cells were isolated from the bone marrow of Lsd1fl/fl and Lsd1fl/fl Mx1Cre animals by FACS-sorting, one week after the final p(I:C) dose. Total RNA from three biological replicates per genotype was extracted and used to hybridize to Affymetrix expression arrays using the Mouse Genome 430 2.0 array platform.
Experiment type
transcription profiling by array 
Contacts
Marc A. Kerenyi <geo@ncbi.nlm.nih.gov>, Marc A Kerenyi, Stuart H Orkin
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-40282.idf.txt
Sample and data relationshipE-GEOD-40282.sdrf.txt
Raw data (1)E-GEOD-40282.raw.1.zip
Processed data (1)E-GEOD-40282.processed.1.zip
Array designA-AFFY-45.adf.txt
Links