E-GEOD-40240 - Expression data from peripheral blood - blood draws at Pre and Post time points of Allergen inhalation challenge (ER and DR)
Released on 20 September 2013, last updated on 30 September 2013
To determine differential gene expression in peripheral blood of asthmatic individuals undergoing allergen inhalation challenge between early responders (ERs) and dual responders (DRs) following allergen inhalation challenge Blood was collected immediately prior to, and two hours after challenge The change in gene expression (post expression minus pre expression) in ERs was compared with the change in gene expression in DRs using age and sex as covariates Preprocessing and probeset filtering were applied to the entire dataset (28 CEL files) using the Factor Analysis for Robust MicroArray Summarization (farms) package in R The Linear Models for MicroArrays (limma) package was used to determine differential gene expression using a Benjamini Hochberg FDR of 10%.
transcription profiling by array
Scott Tebbutt <email@example.com>, Amrit Singh, Cynthia Kanagaratham, Danuta Radzioch, Gabriella Wojewodka, Gail M Gauvreau, J M FitzGerald, Jian Ruan, Juan B De Sanctis, Louis-Philippe Boulet, Masatsugu Yamamoto, Paul M O'Byrne, Sarah H Kam, Scott J Tebbutt
Gene-metabolite expression in blood can discriminate allergen-induced isolated early from dual asthmatic responses. Singh A, Yamamoto M, Kam SH, Ruan J, Gauvreau GM, O'Byrne PM, FitzGerald JM, Schellenberg R, Boulet LP, Wojewodka G, Kanagaratham C, De Sanctis JB, Radzioch D, Tebbutt SJ. , Europe PMC 23844124