E-GEOD-40104 - High-throughput sequencing of Brf1 (Zfp36l1) and Brf2 (Zfp36l2) targets

Status
Released on 30 April 2014, last updated on 1 June 2014
Organism
Mus musculus
Samples (6)
Protocols (3)
Description
We conducted RNA/RNA binding protein immunoprecipitation (RIP) followed by high throughput sequencing to identify mRNA targets of Brf1 (Zfp36l1) and Brf2 (Zfp36l2) in mouse embryonic stem cells. We collected over 190 million sequencing reads and identified many highly enriched protein coding mRNAs. Gene ontology analysis of enriched genes reveals that transcription factors and intercellular signaling proteins account for a large fraction of the targets, many of which are important for pluripotency and differentiation. Identification of Brf1 and Brf2 mRNA targets
Experiment type
RNA-seq of coding RNA 
Contacts
Frederick E Tan <ftan@caltech.edu>, Frederick Tan, Michael Elowitz
Citation
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
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