E-GEOD-39919 - Transcriptional profiling of gastric epithelial cells infected with wild type or arginase-deficient Helicobacter pylori

Status
Released on 15 June 2013, last updated on 2 June 2014
Organism
Homo sapiens
Samples (20)
Array (1)
Protocols (7)
Description
We studied the differential gene expression in the gastric cell line AGS infected with wild type (WT) or arginase gene mutant (rocF) Helicobacter pylori (Hp). We found that the presence of the gene regulates the inflammatory responses in terms of IL8 and MIP-1B. We conclude that arginase may be involved in regulating inflammatory responses which improves the chances of survival against the human immune response. AGS cells were infected for 16 hours with WT, the rocF, or a complemented H. pylori strain (rocF+). The RNA was extracted with TRIzol, treated with DNAse I (both from invitrogen). cRNA was made with the Illumina TotalPrep RNA amplification Kit (ambion AMIL1791).
Experiment type
transcription profiling by array 
Contacts
Jovanny Zabaleta <jzabal@lsuhsc.edu>, David J McGee, Rosa A Sierra, Songhee H Kim
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-39919.idf.txt
Sample and data relationshipE-GEOD-39919.sdrf.txt
Processed data (1)E-GEOD-39919.processed.1.zip
Array designA-MEXP-1171.adf.txt
Links