E-GEOD-39769 - Development of gene expression signatures in testis for plin1-null mice

Status
Released on 26 March 2013, last updated on 31 March 2013
Organism
Mus musculus
Samples (18)
Array (1)
Protocols (6)
Description
We have employed whole genome microarray expression profiling as a discovery platform to identify the effect of plin1 on gene expression during testis development. Testis of day 21, day 40, and day 60 in plin1-null mice and wild type mice were analyzed. Expression of a list genes (including cyp51, prm1, tnp2) from this signature was quantified in the same RNA samples by real-time PCR, confirming the influence of plin1 on testis development. plin1 knockout alteration gene expression in mice testis was measured at different development phases: day 21 meiosis phase, day 40 puberty phase, and day 60 mature phase. There independent experiments were performed at each time (d21, d40, d60).
Experiment type
transcription profiling by array 
Contacts
min chen <chenminchenmin986@163.com>, Min Chen, Ning Li, Qing Y Meng
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-39769.idf.txt
Sample and data relationshipE-GEOD-39769.sdrf.txt
Raw data (1)E-GEOD-39769.raw.1.zip
Processed data (1)E-GEOD-39769.processed.1.zip
Array designA-MEXP-724.adf.txt
Links