E-GEOD-39761 - CD8+ T cell gene expression after different antigen dose stimulations

Status
Released on 1 December 2013, last updated on 2 June 2014
Organism
Homo sapiens
Samples (10)
Array (1)
Protocols (7)
Description
This study aimed to identify the human T cell response toward different antigen doses. Fresh isolated CD8+ T cells from peripheral blood mononuclear cells (PBMCs) were stimulated with influenza M1 peptide-loaded autologous monocyte-derived dendritic cells for 2 weeks. A high M1 peptide antigen dose (10uM on moDC, HD1-5) and an optimal antigen dose (10nM on moDC OD1-5) were used. M1-specific CD8 T cells were tetramer sorted at the end of the 2 weeks. RNA was extracted and analyzed for gene expression using Agilent 8X60K gene expression microarrays. 5 independent samples from 3 different HLA A2+ blood donors.
Experiment type
transcription profiling by array 
Contacts
Yen-Ling Chiu <geo@ncbi.nlm.nih.gov>, Jonathan P Schneck, Yen L Chiu
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-39761.idf.txt
Sample and data relationshipE-GEOD-39761.sdrf.txt
Raw data (1)E-GEOD-39761.raw.1.zip
Processed data (1)E-GEOD-39761.processed.1.zip
Array designA-GEOD-13607.adf.txt
Links