E-GEOD-39707 - Zebrafish whole embryos at 36 hpf treated with DMSO or 5uM 11,12-EET

Status
Released on 27 July 2012, last updated on 24 September 2013
Organism
Danio rerio
Samples (6)
Array (1)
Protocols (8)
Description
In our previous study, we found zebrafish embryos treated with 5uM 11,12-EET (epoxyeicosatrienoic acid) had increased stem cell marker, runx1, expression in the AGM. EET also induced ectopic runx1 expression in the tail. To systematically study how EET regulates gene expression, we performed microarray analysis on EET-treated embryos. Zebrafish whole embryos were synchronized at fertilization. Embryos were grown at 28 degree overnight. 25 embryos per group were treated with DMSO or 5uM 11,12-EET starting from 24 hpf (hour post fertilization) until 36 hpf at 28 degree. The triplicates were from three different clutches of embryos, and split into DMSO v.s. EET for each clutch. EET vs. DMSO
Experiment types
transcription profiling by array, compound treatment design
Contacts
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-39707.idf.txt
Sample and data relationshipE-GEOD-39707.sdrf.txt
Raw data (1)E-GEOD-39707.raw.1.zip
Processed data (1)E-GEOD-39707.processed.1.zip
Array designA-AFFY-38.adf.txt
Links