E-GEOD-39687 - Nitrogen-efficient rice over-expressing alanine aminotransferase

Status
Released on 26 July 2012, last updated on 30 September 2015
Organism
Oryza sativa
Samples (32)
Array (1)
Protocols (7)
Description
Oryza sativa cv. Nipponbare was engineered to over-express a barley alanine aminotransferase (alaAT) gene using the promoter (OsANT1) from a rice aldehyde dehydrogenase gene that expresses in roots. We are using biotechnology to improve the nitrogen use efficiency of rice by over-expressing alaAT in a tissue specific (root) manner. The AlaAT enzyme is a reversible aminotransferase that is linked to both C and N metabolism since it uses pyruvate plus glutamate to produce alanine and 2-oxoglutarate, and visa versa. Wildtype rice (Nipponbare) and three independent OsANT1:HvAlaAT rice transgenic lines (AGR1/7, AGR1/8 and AGR3/8) were grown hydroponically with 5mM NH4+ as the nitrogen source, to the reproductive stage. RNA samples were taken at active tillering, maximum tillering and end-of-tillering stages from root and shoot, at mid-day of the plants' day/night cycle. The RNA from root and shoot at maxiumum tillering was used for microarray analysis. Please read Beatty et al., 2009, Plant Biotechnology Journal 7, pp562-576 for further details..
Experiment type
transcription profiling by array 
Contacts
Perrin Hudson Beatty <pbeatty@ualberta.ca>, Allen G Good, Ashok K Shrawat, Perrin H Beatty, Rebecka T Carroll, Tong Zhu
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-39687.idf.txt
Sample and data relationshipE-GEOD-39687.sdrf.txt
Raw data (2)E-GEOD-39687.raw.1.zip, E-GEOD-39687.raw.2.zip
Processed data (1)E-GEOD-39687.processed.1.zip
Array designA-AFFY-126.adf.txt
Links