E-GEOD-39554 - Expression data from early B cell progenitors including CLP,ProB and PreB of Pax5 knockout and wild type C57Bl6 mice

Status
Released on 22 July 2012, last updated on 6 August 2012
Organism
Mus musculus
Samples (14)
Array (1)
Protocols (8)
Description
we have investigated molecular and functional properties in early B-lineage cells from Pax-5 deficient animals crossed to a B-lineage restricted reporter mouse. Gene expression analysis of ex vivo isolated progenitor cells revealed that Pax-5 deficiency has a minor impact on Bcell specification.By comparison of gene expression patterns in ex vivo isolated Pax-5 and Ebf-1 deficient progenitors, it was possible to identify a set of B-cell restricted genes dependent of Ebf-1 but not Pax-5, supporting the idea that B-cell specification and commitment is controlled by distinct regulatory networks. wt λ5 reporter+ and Pax5ko λ5reporter+ fetal liver from d15 embryos isolated and transplanted into sublethaly irradiated mice. After 4 weeks B220+λ5+ cells were sorted out from the bone marrow of the mice and subjected to microarray analyses
Experiment type
transcription profiling by array 
Contacts
Sasan Zandi <sazandi@gmail.com>, Hong Qian, Jenny Stjernberg, Josefine Åhsberg, Mikael Sigvardsson, Panagiotis Tsapogas
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-39554.idf.txt
Sample and data relationshipE-GEOD-39554.sdrf.txt
Raw data (1)E-GEOD-39554.raw.1.zip
Processed data (1)E-GEOD-39554.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-39554.eSet.r
Links