E-GEOD-38837 - Zbtb20-mediated repression of genes in developing CA1 pyramidal neurons

Status
Released on 11 April 2014, last updated on 2 June 2014
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (5)
Description
The transcriptional repressor Zbtb20 is essential for specification of hippocampal CA1 pyramidal neurons. Moreover, ectopic expression of Zbtb20 is sufficient to transform subicular and retrosplenial areas of D6/Zbtb20S mice to CA1. We used microarrays to identify genes that are repressed by Zbtb20 in developing CA1 pyramidal neurons in the CA1-transformed cortex of D6/Zbtb20S mice. For RNA extraction and hybridization on Affymetrix microarrays, we isolated the CA1-transformed subiculum and retrosplenial cortex from postnatal day 1 D6/Zbtb20S mice, as well as corresponding areas from their wildtype littermates. Total RNA was extracted using the RNeasy Lipid Tissue Mini Kit (Qiagen). Each RNA sample represents a pool of RNA obtained from dissected tissues of seven animals.
Experiment type
transcription profiling by array 
Contacts
Mads Thomassen <mads.thomassen@ouh.fyns-amt.dk>, Jakob V Nielsen, Jens Noraberg, Niels A Jensen, Torben A Kruse
Citation
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-38837.idf.txt
Sample and data relationshipE-GEOD-38837.sdrf.txt
Raw data (1)E-GEOD-38837.raw.1.zip
Processed data (1)E-GEOD-38837.processed.1.zip
Array designA-AFFY-45.adf.txt
Links