E-GEOD-38690 - The ERRalpha metabolic nuclear receptor controls growth of colon cancer cells
Released on 6 June 2013, last updated on 2 June 2014
Estrogen-Related Receptor alpha (ERRα) is a nuclear receptor that acts principally as a regulator of metabolism processes particularly in tissues subjected to high-energy demand. Besides its implication in energy metabolism and mitochondrial biogenesis, ERRα was recently associated with tumorigenesis. Notably, increased expression of ERRα was noted in different cancerous tissues as breast, ovary and colon. However, supplemental studies are required to better understand the role of ERRα in colon carcinoma. We used microarrays to detail gene expression during ERRalpha knockdown in HCT116 cells and identified distinct classes of up and down-regulated genes. Triplicate of HCT116 cell line was infected witn shscrambled(shCTL) and shERRalpha lentivirus and used for RNA extraction and hybridization on Affymetrix microarrays.
transcription profiling by array
Julie Carrier <email@example.com>, André Carpentier, Gérald Bernatchez, Julie C Carrier, Nathalie Rivard, Thomas Lassalle, Véronique Giroux