E-GEOD-38504 - ChIP-on-chip analysis of Ring1B, Ring1A, H2AK119u1 and H3K27me3 in mouse ES cells

Status
Released on 5 June 2012, last updated on 3 May 2014
Organism
Mus musculus
Samples (56)
Arrays (2)
Protocols (8)
Description
ChIP-on-chip analysis was carried out to determine targets of Ring1B, Ring1A, H2AK119u1 and H3K27me3 in mouse ES cells. ChIP-on-chip analysis was carried out using the Mouse Promoter ChIP-on-chip Microarray Set (G4490A, Agilent, Palo Alto, Calif., USA). ESCs were subjected to ChIP assay using specific antibodies. Purified immunoprecipitated and input DNA was subjected to T7 RNA polymerase-based amplification as described previously (van Bakel et al., 2008). Labeling, hybridization and washing were carried out according to the Agilent mammalian ChIP-on-chip protocol (ver.9.0). Scanned images were quantified with Agilent Feature Extraction software under standard conditions.
Experiment type
ChIP-chip by array 
Contacts
Takaho Akiyama Endo <taendo@base.riken.jp>, Haruhiko Koseki, Mitsuhiro Endoh, Takaho A Endo
MIAME
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