7 protocols
AccessionNameType
P-GSE38082-1
bioassay_data_transformation
ID_REF = VALUE = normalized log10 ratio (Cy5/Cy3) representing test/reference
P-GSE38082-7
feature_extraction
Agilent Feature Extraction Software (v 8.5.1.1) was used for background subtraction and LOWESS normalization.
P-GSE38082-6
image_aquisition
Scanned on an Agilent G2565AA scanner. Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1).
P-GSE38082-5
hybridization
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
P-GSE38082-4
labeling
100 ng-input two-round RT/IVT RNA amplification protocol
P-GSE38082-2
grow
Adipose tissues (eWAT) were isolated from the db/db and the lean control (db/+) mice at 10-week of age. The db/db mice are obese, severely hyperglycemic and only modestly hyperinsulinemic at 10 weeks of age.
P-GSE38082-3
nucleic_acid_extraction
Total RNA extracted using Trizol following manufacturer's instructions