7 protocols
AccessionType
bioassay_data_transformation
ID_REF = VALUE = normalized log10 ratio (Cy5/Cy3) representing test/reference
feature_extraction
Agilent Feature Extraction Software (v 8.5.1.1) was used for background subtraction and LOWESS normalization.
image_aquisition
Scanned on an Agilent G2565AA scanner. Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1).
hybridization
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
labeling
100 ng-input two-round RT/IVT RNA amplification protocol
grow
Adipose tissues (eWAT) were isolated from the db/db and the lean control (db/+) mice at 10-week of age. The db/db mice are obese, severely hyperglycemic and only modestly hyperinsulinemic at 10 weeks of age.
nucleic_acid_extraction
Total RNA extracted using Trizol following manufacturer's instructions