E-GEOD-37973 - Discovery of the genes controlled by the second messenger cyclic di-GMP in Mycobacterium tuberculosis

Status
Released on 9 October 2013, last updated on 2 June 2014
Organism
Mycobacterium tuberculosis
Samples (5)
Array (1)
Protocols (7)
Description
Cyclic di-GMP (c-di-GMP) is a ubiquitous second messenger that regulates many biological processes in bacteria. The genome in Mycobacterium tuberculosis encodes a single copy of the diguanylate cyclase gene (dgc) responsible for c-di-GMP synthesis. To determine the role of c-di-GMP signaling in M. tuberculosis, the mutant strain of Δdgc was generated in the virulent H37Rv strain. We used whole genome microarray expression profiling as a discovery platform to identify the genes controlled by c-di-GMP in M. tuberculosis, providing molecular proof for the phenotypes modulated by the signaling. Wild-type H37Rv and Δdgc cultures were analyzed under aerobic conditions or in an in vitro dormancy model. Bacteria were collected at OD600 =1.3 for the aerobic cultures and upon the beginning of anaerobiosis for the cultures in the dormancy model. One culture for each experiment was assayed except for Δdgc under anaerobiosis (2 independent cultures).
Experiment type
transcription profiling by array 
Contacts
Haihong Fang, Yuzhi Hong
Citation
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-37973.idf.txt
Sample and data relationshipE-GEOD-37973.sdrf.txt
Raw data (1)E-GEOD-37973.raw.1.zip
Processed data (1)E-GEOD-37973.processed.1.zip
Array designA-GEOD-15565.adf.txt
Links