7 protocols
AccessionNameType
P-GSE37949-1
bioassay_data_transformation
ID_REF = VALUE = MAS5.0 signal intensity ABS_CALL = indicating whether the transcript was present (P), absent (A), or marginal (M) DETECTION P-VALUE = p-value that indicates the significance level of the detection call
P-GSE37949-7
feature_extraction
The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling as the normalization method.
P-GSE37949-6
image_aquisition
Service by The University of Hong Kong (HKU) Genome Research Centre (protocol attached to the Series).
P-GSE37949-5
hybridization
Service by The University of Hong Kong (HKU) Genome Research Centre (protocol attached to the Series).
P-GSE37949-4
labeling
Service by The University of Hong Kong (HKU) Genome Research Centre (protocol attached to the Series).
P-GSE37949-3
nucleic_acid_extraction
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
P-GSE37949-2
grow
CD90+ and CD90- cells were sorted from KYSE-140 ESCC cells using the flow cytometry cell sorting technique.