7 protocols
AccessionType
bioassay_data_transformation
ID_REF = VALUE = MAS5.0 signal intensity ABS_CALL = indicating whether the transcript was present (P), absent (A), or marginal (M) DETECTION P-VALUE = p-value that indicates the significance level of the detection call
feature_extraction
The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling as the normalization method.
image_aquisition
Service by The University of Hong Kong (HKU) Genome Research Centre (protocol attached to the Series).
hybridization
Service by The University of Hong Kong (HKU) Genome Research Centre (protocol attached to the Series).
labeling
Service by The University of Hong Kong (HKU) Genome Research Centre (protocol attached to the Series).
nucleic_acid_extraction
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
grow
CD90+ and CD90- cells were sorted from KYSE-140 ESCC cells using the flow cytometry cell sorting technique.