E-GEOD-37470 - Large Granular Lymphocyte Leukemia Mesenchymal Stem Cells

Status
Released on 20 April 2013, last updated on 2 June 2014
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (7)
Description
The bone marrow microenvironment in Large Granular Lymphocyte Leukemia (LGLL) patients has been unexplored for it’s role in the development of cytopenias, which lead to complications resulting in the most prominent causes of morbidity and mortality. We used microarrays on primary mesenchymal stem cell (MSC) cultures isolated from bone marrow aspirates from LGLL patients to identify genetic programs that may lead to the observed profibrotic and extrinsically senescent phenotype. Isolated primary MSC cultures were maintained under reduced oxygen conditions (2%). All cultures displayed trilineage pluripotency (adipogenesis, osteogenesis, and chondrogenesis). For comparison, normal MSC cultures in early passage (p2-3; same number of population doublings as the LGLL MSCs) and later passage (p7-9; same time spent in culture as the LGLL MSCs) are included.
Experiment type
transcription profiling by array 
Contacts
Adam Mailloux <adam.mailloux@moffitt.org>, Adam W Mailloux, Cody Wei, Gregory Bloom, John M Bennett, Ling Zhang, Lubomir Sokol, Lynn Moscinski, Pearlie-K Epling-Burnette, Sean Yoder, Steven Enkemann, Thomas P Loughran
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-37470.idf.txt
Sample and data relationshipE-GEOD-37470.sdrf.txt
Raw data (1)E-GEOD-37470.raw.1.zip
Processed data (1)E-GEOD-37470.processed.1.zip
Array designA-AFFY-44.adf.txt
Links