E-GEOD-37470 - Large Granular Lymphocyte Leukemia Mesenchymal Stem Cells
Released on 20 April 2013, last updated on 2 June 2014
The bone marrow microenvironment in Large Granular Lymphocyte Leukemia (LGLL) patients has been unexplored for it’s role in the development of cytopenias, which lead to complications resulting in the most prominent causes of morbidity and mortality. We used microarrays on primary mesenchymal stem cell (MSC) cultures isolated from bone marrow aspirates from LGLL patients to identify genetic programs that may lead to the observed profibrotic and extrinsically senescent phenotype. Isolated primary MSC cultures were maintained under reduced oxygen conditions (2%). All cultures displayed trilineage pluripotency (adipogenesis, osteogenesis, and chondrogenesis). For comparison, normal MSC cultures in early passage (p2-3; same number of population doublings as the LGLL MSCs) and later passage (p7-9; same time spent in culture as the LGLL MSCs) are included.
transcription profiling by array
Adam Mailloux <email@example.com>, Adam W Mailloux, Cody Wei, Gregory Bloom, John M Bennett, Ling Zhang, Lubomir Sokol, Lynn Moscinski, Pearlie-K Epling-Burnette, Sean Yoder, Steven Enkemann, Thomas P Loughran