E-GEOD-37458 - Transcription profiling by array of ventricles from mice with homozygous knockdown expression of the vesicular acetylcholine transporter (VAChT) and wild type controls

Status
Released on 14 November 2012, last updated on 30 April 2015
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (8)
Description
VAChT KDHOM mice have a 70% decrease in the vesicular acetylcholine transporter (VAChT) and this leads to a systemic decrease in ACh release and cardiac dysfunction. We used this microarray to determine whether there were transcriptional alterations in the hearts of mutant mice as a result of this long-term decrease in cholinergic tone. We isolated whole hearts from 3-month-old WT and VAChT KD mice and used them for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain homogenous populations for each sample and, as such, pooled together two RNA samples to generate one microarray sample.
Experiment type
transcription profiling by array 
Contacts
David E Carter <dcarter@robarts.ca>, Gros Robert, Prado Marco A.M.
Citation
An analysis of the myocardial transcriptome in a mouse model of cardiac dysfunction with decreased cholinergic neurotransmission. Roy A, Lara A, Guimar�es D, Pires R, Gomes ER, Carter DE, Gomez MV, Guatimosim S, Prado VF, Prado MA, Gros R.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-37458.idf.txt
Sample and data relationshipE-GEOD-37458.sdrf.txt
Raw data (1)E-GEOD-37458.raw.1.zip
Processed data (1)E-GEOD-37458.processed.1.zip
Array designA-AFFY-130.adf.txt
Links