E-GEOD-37458 - Transcription profiling by array of ventricles from mice with homozygous knockdown expression of the vesicular acetylcholine transporter (VAChT) and wild type controls
Released on 14 November 2012, last updated on 30 April 2015
VAChT KDHOM mice have a 70% decrease in the vesicular acetylcholine transporter (VAChT) and this leads to a systemic decrease in ACh release and cardiac dysfunction. We used this microarray to determine whether there were transcriptional alterations in the hearts of mutant mice as a result of this long-term decrease in cholinergic tone. We isolated whole hearts from 3-month-old WT and VAChT KD mice and used them for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain homogenous populations for each sample and, as such, pooled together two RNA samples to generate one microarray sample.
transcription profiling by array
David E Carter <firstname.lastname@example.org>, Gros Robert, Prado Marco A.M.
An analysis of the myocardial transcriptome in a mouse model of cardiac dysfunction with decreased cholinergic neurotransmission. Roy A, Lara A, Guimar�es D, Pires R, Gomes ER, Carter DE, Gomez MV, Guatimosim S, Prado VF, Prado MA, Gros R.