E-GEOD-37357 - Topoisomerase I cleavage complexes preferentially down-regulate ubiquitin- and RNA degradation-related transcripts in relationship to gene length, exon-intron junctions and p53-dependent up-regulation of miR-142-3p [miRNA]

Status
Released on 17 April 2013, last updated on 2 June 2014
Organism
Homo sapiens
Samples (7)
Array (1)
Protocols (7)
Description
DNA topoisomerase I (Top1) is required for transcription as it relaxes positive and negative supercoils by forming transient Top1 cleavage complexes (Top1cc) up- and down-stream of transcription complexes. However, Top1cc can also be trapped by endogenous DNA lesions and by camptothecin (CPT) and its anticancer derivatives, which results in transcription blocks. Here, we undertook a genome-wide analysis of the effects of CPT on gene expression at exon resolution. We tested the impact of Top1 inhibition on miRNA expression at the genome-wide level in human colon carcinoma HCT116. The miRNA of cells treated with camptothecin (CPT) for were measured at several timepoints with Agilent Human miRNA Microarray V3 (8x15K).
Experiment type
transcription profiling by array 
Contacts
Michael Ryan <mryan@insilico.us.com>, Barry R Zeeberg, Hongfang Liu, Kurt W Kohn, Mike C Ryan, Stéphanie Solier, Sudhir Varma, Yves Pommier
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-37357.idf.txt
Sample and data relationshipE-GEOD-37357.sdrf.txt
Raw data (1)E-GEOD-37357.raw.1.zip
Processed data (1)E-GEOD-37357.processed.1.zip
Array designA-GEOD-10850.adf.txt
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