E-GEOD-37306 - CGH of Plasmodium falciparum selected with DSM1

Status
Released on 1 May 2013, last updated on 2 June 2014
Organism
Plasmodium falciparum
Samples (16)
Arrays (2)
Protocols (11)
Description
Continual challenge of 107 ARMD Dd2 parasites with 0.3µM DSM1, a novel dihydroorotate dehydrogenase (DHODH) inhibitor, reproducibly generated ~5-fold resistance and 3-fold amplification of 30-100Kb DNA, always including the DHODH gene. Subsequent 3-10µM DSM-1 pressure selected for ~10-fold amplification of the copy number variant (CNV), and over 100-fold resistance. Target-specific and genome-wide DNA sequencing revealed no additional mutations contributing to DSM1 resistance. Each DSM1-resistant clone is compared to the Dd2 parent reference sample.
Experiment type
comparative genomic hybridization by array 
Contacts
MIAME
PlatformsProtocolsFactorsProcessedRaw
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