E-GEOD-3680 - Beta-naphthoflavone treated worms

Status
Submitted on 25 November 2005, released on 25 November 2005, last updated on 27 March 2012
Organism
Caenorhabditis elegans
Samples (6)
Arrays (3)
Protocols (2)
Description
The soil nematode Caenorhabditis elegans is one of the simplest animals having the status of a laboratory model. Its genome contains 80 cytochrome P450 genes (CYP). In order to study CYP gene expression in C. elegans mixed stages and synchronized hermaphrodites were exposed to 18 known xenobiotic cytochrome P450 inducers. Messenger RNA expression was detected by DNA arrays and semiquantitative RT-PCR. Using subfamily-specific primers, a pooled set of exon-rich CYP fragments could be amplified. In this way it was possible to systematically check the influence of different inducers on CYP expression at the same time. The well-known CYP1A inducers beta-naphthoflavone, PCB52, and lansoprazol were the most active and in particular they strongly induced almost all CYP35 isoforms. A few number of further CYP forms were found to be inducible by other xenobiotics like phenobarbital, atrazine, and clofibrate. In addition, a transgenic C. elegans line expressing GFP under control of the CYP35A2 promoter showed a strong induction of the fusion by beta-naphthoflavone in the intestine. Copyright 2001 Academic Press. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Computed
Experiment type
unknown experiment type 
Contacts
Citation
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-3680.idf.txt
Sample and data relationshipE-GEOD-3680.sdrf.txt
Processed data (1)E-GEOD-3680.processed.1.zip
Array designsA-GEOD-2646.adf.txt, A-GEOD-2655.adf.txt, A-GEOD-3097.adf.txt
Links