E-GEOD-36565 - Whole genome expression after modulation of miR-30a expression

Status
Released on 22 March 2013, last updated on 2 June 2014
Organism
Homo sapiens
Samples (10)
Array (1)
Protocols (8)
Description
A subset of breast cancer cells displays increased ability to self-renew and reproduce breast cancer heterogeneity (so called, breast tumour-initiating cells or BT-ICs). As microRNAs (miRNAs) control developmental programs in stem cells, BT-ICs may also rely on specific miRNA profiles for their sustained activity. We analyzed miRNA expression in a model of putative BT-ICs and found that miR-30 family regulates growth under “stemness” conditions. A target screening revealed that miR-30 family modulates the expression of apoptosis and proliferation-related genes. The importance of miR-30 in tumour progression and breast cancer stemness was demonstrated in vivo using a mouse mammary cancer model. This is the first analysis of target prediction in a whole family of microRNAs potentially involved in survival of putative BT-ICs. Total RNA from cells transfected with Pre-miR-30 and KD-miR-30 family and control KD-miR-159 was extracted and reverse-transcribed and hybrized on HT12 Human bead chips
Experiment type
transcription profiling by array 
Contacts
Citation
MicroRNA miR-30 family regulates non-attachment growth of breast cancer cells. Ouzounova M, Vuong T, Ancey PB, Ferrand M, Durand G, Le-Calvez Kelm F, Croce C, Matar C, Herceg Z, Hernandez-Vargas H. , Europe PMC 23445407
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-36565.idf.txt
Sample and data relationshipE-GEOD-36565.sdrf.txt
Processed data (1)E-GEOD-36565.processed.1.zip
Array designA-GEOD-10558.adf.txt
Links