E-GEOD-36474 - Comparison of bone-marrow mesenchymal stromal cells from multiple myeloma patients and healthy donors

Status
Released on 21 March 2013, last updated on 2 June 2014
Organism
Homo sapiens
Samples (7)
Array (1)
Protocols (7)
Description
It is now well established that bone marrow (BM) constitutes a microenvironment required for differentiation. Bone marrow mesenchymal stromal cells (BM-MSCs) strongly support MM cell growth, by producing a high level of Interleukin-6 (IL-6), a major MM cell growth factor. BM-MSCs also support osteoclastogenesis and angiogenesis. Previous studies have suggested that the direct (VLA-4, VCAM-1, CD44, VLA-5, LFA-1, syndecan-1,…) and indirect interactions (soluble factors) between MM plasma cells and BM-MSCs result in constitutive abnormalities in BM-MSCs. In particular, MM BM-MSCs express less CD106 and fibronectin and more DKK1, IL-1β and TNF-α as compared with normal BM-MSCs. In order to gain a global view of the differences between BM-MSCs from MM patients and healthy donors, we used gene expression profiling to identify genes associated to the transformation of MM BM-MSCs. BM-MSCs were isolated from 3 healthy donors and 4 untreated multiple myeloma patients. Total RNA from BM-MSCs was exctracted and hybridyzed on Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array. Amplification, hybridization and scanning were done according to standard Affymetrix protocols (www.affymetrix.com). CEL files were normalized with RMA method.
Experiment type
transcription profiling by array 
Contacts
Thibaud André <thibaud.andre@bordet.be>, B Stamatopoulos, C De Bruyn, D Bron, K Pieters, L Lagneaux, N Meuleman, T André
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-36474.idf.txt
Sample and data relationshipE-GEOD-36474.sdrf.txt
Raw data (1)E-GEOD-36474.raw.1.zip
Processed data (1)E-GEOD-36474.processed.1.zip
Array designA-AFFY-44.adf.txt
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