E-GEOD-36252 - X-chromosome inactivation in monkey embryos and pluripotent stem cells

Status
Released on 31 August 2012, last updated on 1 October 2012
Organism
Macaca mulatta
Samples (21)
Array (1)
Protocols (8)
Description
In this study, we explored x-inactivation in monkey embryos (ICM and TE separately) and pluripotent stem cells (IVF derived ES, SCNT-derived ES and monkey iPS) To elucidate x-inactivation in experimentally reprogrammed pluripotent cells, we derived pluripotent stem cells by both SCNT and iPS approaches from same parental skin fibroblasts. We also compared gene patterns of those cells to IVF-derived counterpart. The transcriptomes of rhesus monkey embryonic stem cell lines derived by both SCNT (CRES) and iPS (RiPS) from same monkey skin fibroblasts were compared each other. Both experimentally reprogrammed cells were also compared with IVF-derived counterpart (ORMES23). Finally, the adult somatic skin fibroblasts were analyzed. Three biological replicates of each cell line (A, B, C) were analyzed.
Experiment type
transcription profiling by array 
Contacts
Shoukhrat Mitalipov <mitalipo@ohsu.edu>, Hathaitip Sritanaudomchai, Hong Ma, Masahito Tachibana
Citation
X-chromosome inactivation in monkey embryos and pluripotent stem cells. Tachibana M, Ma H, Sparman ML, Lee HS, Ramsey CM, Woodward JS, Sritanaudomchai H, Masterson KR, Wolff EE, Jia Y, Mitalipov SM. , Europe PMC 22935618
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-36252.idf.txt
Sample and data relationshipE-GEOD-36252.sdrf.txt
Raw data (1)E-GEOD-36252.raw.1.zip
Processed data (1)E-GEOD-36252.processed.1.zip
Array designA-AFFY-145.adf.txt
R ExpressionSetE-GEOD-36252.eSet.r
Links