E-GEOD-3566 - Transcription profiling by array of Drosophila Kc167 cells with RNAi knockdown of Enigma, a mitochondrial protein with homology to acyl-CoA dehydrogenases

Status
Released on 31 July 2007, last updated on 24 March 2014
Organism
Drosophila melanogaster
Samples (6)
Array (1)
Protocols (4)
Description
5 day RNAi treatment to knockdown Enigma, CG9006, a Drosophila mitochondrial protein with homology to acyl-CoA dehydrogenases. Experiment Overall Design: Kc-167 cells, 5 day treatment with Enigma-specific RNAi or beta-lactamase control RNAi. Specifics:1.6X10^6 cells in 0.5mL of serum-free Sang's M3 media incubated for 1 hour with 20ug of dsRNA, subsequently equal volume of M3 media containing 10% serum was added. Cells harvested after 5 days. RNAi efficiency measured by immunoblot for Enigma.
Experiment types
transcription profiling by array, cellular modification
Contact
Citation
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-3566.idf.txt
Sample and data relationshipE-GEOD-3566.sdrf.txt
Raw data (1)E-GEOD-3566.raw.1.zip
Processed data (1)E-GEOD-3566.processed.1.zip
Array designA-AFFY-17.adf.txt
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