E-GEOD-35496 - ChIP-Seq of Sox2 and Brn2 in ESCs, NPCs, and differentiating ESCs

Status
Released on 20 February 2013, last updated on 14 March 2013
Organism
Mus musculus
Samples (30)
Protocols (16)
Description
We analyzed the genome-wide binding of Sox2 and POU factor partner factors, Oct4 in ESCs (using published datasets PMID:18692474 and GSM307137, GSM307154, GSM307155) and Brn2 in NPCs. We found that Sox2 and Oct4 co-occupied a large subset of promoters and enhancers in ESCs, but that Sox2 and Brn2 co-occupy predominantly enhancers. Further, we overexpressed Brn2 in differentiating ESCs and showed that ectopic Brn2 recruited Sox2 to NPC-specific targets, resulting in skewed differentiation towards the neural lineage. Examination of transcription factor binding in ESCs, NPCs, and differentiating ESCs by ChIP-Seq.
Experiment type
ChIP-seq 
Contacts
Michael Lodato <lodato@wi.mit.edu>, Laurie A Boyer, Michael A Lodato, Rudolf Jaenisch
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
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