E-GEOD-35460 - Gene expression profiling data from sooty mangabeys treated with interferon alpha

Status
Released on 9 July 2012, last updated on 17 July 2012
Organism
Cercocebus atys
Samples (47)
Array (1)
Protocols (8)
Description
In contrast to pathogenic HIV and SIV infection of humans and macaques, SIV infection of sooty mangabeys (SMs) is typically non-pathogenic despite high virus replication. A key feature of primary SIV infection of SMs is a strong type I interferon (IFN-I) response, characterized by massive up-regulation of interferon-stimulated genes (ISG), followed by rapid resolution during the acute-to-chronic phase transition and establishment of an immune quiescent state that persists throughout the chronic infection. Based on these observations we hypothesized that low levels of IFN-I signaling may be instrumental in preventing chronic immune activation and disease progression in SIV-infected SMs. We used microarrays to characterize gene expression changes induced by IFNalpha treatment. To directly assess the effects of an experimentally-induced augmentation of IFN-I signaling in chronically SIV-infected SMs, we administered recombinant rhesus macaque IFNalpha2-IgFc (rmIFNα2) to eight naturally SIV-infected SMs weekly for 16 weeks and longitudinally monitored viral load, lymphocyte counts, immune activation, SIV-specific CD8+ T-cell responses, and gene expression profile. Administration of rmIFNα2 was bioactive in vivo with gene expression profiling revealing a strong upregulation of numerous ISGs in the blood of treated animals.
Experiment type
transcription profiling by array 
Contacts
Gregory K Tharp <gktharp@emory.edu>, Benton O Lawson, Chloe Schlicter, Francois Villinger, Greg K Tharp, Guido Silvestri, Obaede Rend, Steven E Bosinger, Thomas H Vanderford
Citation
Treatment of SIV-infected sooty mangabeys with a type-I IFN agonist results in decreased virus replication without inducing hyperimmune activation. Vanderford TH, Slichter C, Rogers KA, Lawson BO, Obaede R, Else J, Villinger F, Bosinger SE, Silvestri G. , Europe PMC 22550346
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-35460.idf.txt
Sample and data relationshipE-GEOD-35460.sdrf.txt
Raw data (2)E-GEOD-35460.raw.1.zip, E-GEOD-35460.raw.2.zip
Processed data (1)E-GEOD-35460.processed.1.zip
Array designA-AFFY-145.adf.txt
R ExpressionSetE-GEOD-35460.eSet.r
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