E-GEOD-3462 - Triiodothyronine Treatment: Effects on vastus lateralis skeletal muscle
Submitted on 16 October 2005, released on 18 October 2005, last updated on 18 October 2011
Healthy men received 75 5g/d of T3 for 14 days. Microbiopsies of vastus lateralis skeletal muscle were performed before and after the treatment. For microarray experiments, we used samples from five subjects. After amplification of total RNA (Wang et al. 2000a), fluorescently labeled cDNA was prepared from each experimental sample. For each subject, probes from basal and T3 treatment conditions labeled with cyanine (Cy) 3 or Cy5 dyes were hybridized to cDNA microarray. After a filtering procedure to eliminate bad-quality spots and background correction, log2 transformed data for each experiment were normalized and centered to the mean. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Using regression correlation
unknown experiment type
Stanford Microarray Database <email@example.com>, Karine Clement
In vivo regulation of human skeletal muscle gene expression by thyroid hormone. Clément K, Viguerie N, Diehn M, Alizadeh A, Barbe P, Thalamas C, Storey JD, Brown PO, Barsh GS, Langin D.