E-GEOD-3433 - Transcription profiling of mouse strains A/J versus C57BL/6J during diet-induced obesity

Status
Submitted on 11 October 2005, released on 6 November 2008, last updated on 27 March 2012
Organism
Mus musculus
Samples (4)
Array (1)
Description
The effects of high-fat (HF) feeding on the gene expression in the small intestine were examined using obesity-resistant A/J mice and obesity-sensitive C57BL/6J (B6) mice. Both strains of mice were maintained on low-fat (LF, 5% fat) or HF (30% fat) diets for two weeks. Oligonucleotide microarray and semi-quantitative RT-PCR analysis revealed that lipid metabolism-related genes including mitochondrial acyl-CoA thioesterase 1, pyruvate dehydrogenase kinase 4, and NADP+-dependent cytosolic malic enzyme were up-regulated by more than 2-fold in both strains of mice. The up-regulated gene expression levels were significantly higher in A/J mice than in B6 mice, suggesting the active lipid metabolism in the small intestine of A/J mice. Experiment Overall Design: Mice were maintained on the LF or HF diet for two weeks. For each group (n=6), pooled total RNA from two mice of average body weight was used for the analysis.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Differential regulation of intestinal lipid metabolism-related genes in obesity-resistant A/J vs. obesity-prone C57BL/6J mice. Hidehiko Kondo, Yoshihiko Minegishi, Yumiko Komine, Takuya Mori, Ichiro Matsumoto, Keiko Abe, Ichiro Tokimitsu, Tadashi Hase, Takatoshi Murase.
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-3433.idf.txt
Sample and data relationshipE-GEOD-3433.sdrf.txt
Processed data (1)E-GEOD-3433.processed.1.zip
Array designA-AFFY-36.adf.txt
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