E-GEOD-34136 - Influence of intrauterine low protein diet on ductal morphogenesis of the rat mammary gland
Released on 24 June 2014, last updated on 28 June 2014
Following intrauterine low protein diet, the lumbar mammary gland of control and low protein rats were removed for further analysis via genarray on day 21 and day 28 postpartum. Virgin female Wistar rats came from Charles River. Throughout pregnancy the NP rats received 25g/d of Altromin C1000 containing 17.0% protein, while LP animals were fed 25g/d of Altromin C1003 containing 8.8% protein. At birth, litter size was reduced to 6 females in both groups, which were left to suckle for 21 days. During lactation (d1-d21) and after weaning (d21-d28) NP and LP animals were fed a standard chow and tap water ad libitum. At d21 and d28 3 dams were sacrificed under isofluran narcosis. The lumbar mammary gland (4th and 5th pair) were removed and cleared of visible lymph nodes before snap-freezing in liquid nitrogen.
transcription profiling by array
Fabian Fahlbusch <email@example.com>, Andrea Hartner, Carlos Menendez-Castro, Fabian B Fahlbusch, Hanna Huebner, Helmuth-Guenther Doerr, Ilona Winterfeld, Lea Beinder, Manfred Rauh, Matthias Ruebner, Nina Faehrmann, Rainer Wachtveitl, Stephanie C Noegel, Wolfgang Rascher
Detection of Expressional Changes Induced by Intrauterine Growth Restriction in the Developing Rat Mammary Gland via Exploratory Pathways Analysis. Beinder L, Faehrmann N, Wachtveitl R, Winterfeld I, Hartner A, Menendez-Castro C, Rauh M, Ruebner M, Huebner H, Noegel SC, Doerr HG, Rascher W, Fahlbusch FB. , Europe PMC 24955840