E-GEOD-33607 - Expression data from mouse B16-F10 cells exposed to hypoxic conditions in vitro

Status
Released on 11 November 2011, last updated on 25 June 2012
Organism
Mus musculus
Samples (21)
Array (1)
Protocols (8)
Description
Analysis of gene expression profile of B16-F10 murine melanoma cells exposed to hypoxic conditions (1% oxygen) or hypoxia mimicry (cobalt chloride) for 24 hours. Gene expression profiles were analyzed using MG-U74Av2 oligonucleotide microarrays. Data analysis revealed 2541 probesets (FDR<5%) for 1% oxygen experiment and 364 probesets (FDR<5%) for cobalt chloride, that showed differences in expression levels. Analysis of hypoxia-regulated genes (1% O2) by stringent Family-Wise Error Rate estimation indicated 454 significantly changed transcripts (p<0.05). The most upregulated genes were Lgals3, Selenbp1, Nppb (more than ten-fold increase). Both hypoxia and hypoxia-mimicry induced HIF-1 regulated genes. However, unsupervised analysis (Singular Value Decomposition) revealed distinct differences between gene expression induced by these two experimental conditions. We investigated transcriptional activity of B16-F10 murine melanoma cells cultured for 24h under hypoxic (nominal 1% oxygen; 9 experimental samples and 6 controls) and hypoxia-mimicking conditions (cobalt chloride, 100 μM or 200 μM, 2 samples each and 2 controls).
Experiment type
transcription profiling by array 
Contacts
Michal Swierniak <geo@ncbi.nlm.nih.gov>, Aleksander Sochanik, Joanna Jazowiecka-Rakus, Magdalena Olbryt, Michał Jarząb
Citation
Gene expression profile of B 16(F10) murine melanoma cells exposed to hypoxic conditions in vitro. Olbryt M, Jarzab M, Jazowiecka-Rakus J, Simek K, Szala S, Sochanik A.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-33607.idf.txt
Sample and data relationshipE-GEOD-33607.sdrf.txt
Raw data (1)E-GEOD-33607.raw.1.zip
Processed data (1)E-GEOD-33607.processed.1.zip
Array designA-AFFY-6.adf.txt
R ExpressionSetE-GEOD-33607.eSet.r
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