E-GEOD-33607 - Expression data from mouse B16-F10 cells exposed to hypoxic conditions in vitro
Released on 11 November 2011, last updated on 25 June 2012
Analysis of gene expression profile of B16-F10 murine melanoma cells exposed to hypoxic conditions (1% oxygen) or hypoxia mimicry (cobalt chloride) for 24 hours. Gene expression profiles were analyzed using MG-U74Av2 oligonucleotide microarrays. Data analysis revealed 2541 probesets (FDR<5%) for 1% oxygen experiment and 364 probesets (FDR<5%) for cobalt chloride, that showed differences in expression levels. Analysis of hypoxia-regulated genes (1% O2) by stringent Family-Wise Error Rate estimation indicated 454 significantly changed transcripts (p<0.05). The most upregulated genes were Lgals3, Selenbp1, Nppb (more than ten-fold increase). Both hypoxia and hypoxia-mimicry induced HIF-1 regulated genes. However, unsupervised analysis (Singular Value Decomposition) revealed distinct differences between gene expression induced by these two experimental conditions. We investigated transcriptional activity of B16-F10 murine melanoma cells cultured for 24h under hypoxic (nominal 1% oxygen; 9 experimental samples and 6 controls) and hypoxia-mimicking conditions (cobalt chloride, 100 μM or 200 μM, 2 samples each and 2 controls).
transcription profiling by array
Michal Swierniak <firstname.lastname@example.org>, Aleksander Sochanik, Joanna Jazowiecka-Rakus, Magdalena Olbryt, Michał Jarząb
Gene expression profile of B 16(F10) murine melanoma cells exposed to hypoxic conditions in vitro. Olbryt M, Jarzab M, Jazowiecka-Rakus J, Simek K, Szala S, Sochanik A.