E-GEOD-33292 - Expression data from WT DN3, TCF-1-deficient DN3 thymocytes, and T cell lymphomas in TCF-1-deficient animals

Released on 25 October 2012, last updated on 21 September 2015
Mus musculus
Samples (12)
Array (1)
Protocols (8)
TCF-1 is an HMG family transcription factor which is known to be critical for T cell development. We discovered that it has a unique role in suppressing malignant transformation of developing thymocytes at early stages. We identified ID2 and LEF-1 as key TCF-1 target genens in tumor suppression. We used microarrays to detect gene expression changes in WT and TCF-1 deficient DN3 thymocytes as well as T cell lymphoma cells developed in TCF-1 KO mice. DN3 thymocytes were directly sorted from WT or TCF-1 KO mice. T cell lymphoma blast cells were also sorted from TCF-1 KO mice that developed the disease. RNA was extracted and hybridized to GeneChip Mouse GENE 1.0 ST arrays (Affymetrix).
Experiment type
transcription profiling by array 
The TCF-1 and LEF-1 Transcription Factors Have Cooperative and Opposing Roles in T Cell Development and Malignancy. Yu S, Zhou X, Steinke FC, Liu C, Chen SC, Zagorodna O, Jing X, Yokota Y, Meyerholz DK, Mullighan CG, Knudson CM, Zhao DM, Xue HH.
Investigation descriptionE-GEOD-33292.idf.txt
Sample and data relationshipE-GEOD-33292.sdrf.txt
Raw data (1)E-GEOD-33292.raw.1.zip
Processed data (1)E-GEOD-33292.processed.1.zip
Array designA-AFFY-130.adf.txt