E-GEOD-3255 - Gene Expression Profiles with Common Translocations in Acute Myeloid Leukemia (AML) using SAGE

Status
Released on 30 November 2005, last updated on 27 March 2012
Organism
Homo sapiens
Samples (22)
Protocols (1)
Description
SAGE was used to profile gene expression in leukemic myeloid progenitor cells of AML patients with t(8;21), t(15;17), t(9;11) and inv(16). Keywords: SAGE analysis of gene expression in leukemic myeloid progenitor cells isolated from bone marrow Bone marrow cells from 4 patients with de novo t(9;11), 3 with treatment-related t(9;11), and 5 each with inv(16), t(15;17), t(8;21) were used for the study. Each sample contained at least 75% affected cells with no other chromosome abnormalities at cytogenetic level. Mononuclear cells were purified from bone marrow cells for each sample, and CD15+ myeloid progenitor cells were isolated from these mononuclear cells. Poly A+ RNA were isolated from CD15+ myeloid progenitor cells of each sample for SAGE tag collection.
Experiment type
transcription profiling by SAGE 
Contacts
San Ming Wang <swang1@northwestern.edu>, Eric Green, Gerrard Bouffard, Guolin Zhou, Janet D Rowley (Correspondence), Jeffery Trent, Jianjun Chen, Justin Kline, Mark Minden, Masha Kocherginsky, Miao Sun, Michelle LeBeau, Neelmini Emmanuel, Nimanthi Jayathilaka, Ozden Ozer, Paul Liu, Richard Larson, Run Z Shi, San M Wang (Correspondence), Sanggyu Lee, Stefan Bohlander, Theodore Karrison, Xijin Ge, Yeong C Kim
Citation
Gene expression profiles in acute myeloid leukemia with common translocations using SAGE. Lee S, Chen J, Zhou G, Shi RZ, Bouffard GG, Kocherginsky M, Ge X, Sun M, Jayathilaka N, Kim YC, Emmanuel N, Bohlander SK, Minden M, Kline J, Ozer O, Larson RA, LeBeau MM, Green ED, Trent J, Karrison T, Liu PP, Wang SM, Rowley JD. , Europe PMC 16418266
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
Files
Investigation descriptionE-GEOD-3255.idf.txt
Sample and data relationshipE-GEOD-3255.sdrf.txt
Processed data (1)E-GEOD-3255.processed.1.zip
Links