E-GEOD-32083 - Transcriptomics of the traditional Japanese medicine juzentaihoto (JTX) on large intestines on germfree mice

Status
Released on 13 September 2011, last updated on 2 May 2014
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (8)
Description
Juzehtaihoto, a Japanese traditional medicine has been used for the treatment of various kinds of diseases or disorders in an enteric-flora dependent manner. Here, we performed transcriptome analysis using Affymetrix GeneChip on large intestine (LI) of germ-free (GF) and specific pathogen free (SPF) mice of IQI, an inbred strain established from ICR, and BALB/c SPF mice. Male germ-free (GF) and specific pathogen-free (SPF) mice of IQI and BALB/c strains were bred and maintained in the laboratory of Central Institute for Experimental Animals (CIEA, Kawasaki, Kanagwa, Japan). GF mice were housed in a Trexler-type flexible film isolator in a standard germ free state and screened on a weekly basis for germ-free status by sampling feces sterilely and culturing on MRS-agar plates under aerobic and anaerobic conditions. All the GF, SPF, ex-GF mice were kept in a 12: 12-h light/dark cycle and at a temperature of 22±2°C. Nine week old mice (n=3) were sacrificed by cervical dislocation and the intestines were dissected. comparison of the mice with or without enteric flora; comparison of the mice of different strains
Experiment type
transcription profiling by array 
Contacts
Masahiro Yamamoto <hirokoma@h.email.ne.jp>, Kaori Munakata, Kenji Watanabe
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-32083.idf.txt
Sample and data relationshipE-GEOD-32083.sdrf.txt
Raw data (1)E-GEOD-32083.raw.1.zip
Processed data (1)E-GEOD-32083.processed.1.zip
Array designA-AFFY-6.adf.txt
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