E-GEOD-31665 - Role of Cofilin 1 pathway and actin dynamics in nuclear retinoid receptor function

Released on 25 August 2011, last updated on 7 September 2011
Homo sapiens
Samples (12)
Array (1)
Protocols (10)
We have investigated the role of actin dynamics and the effect of actin cytoskeleton modifying agents on retinoid receptor-mediated transactivation. Using Nef, an actin modifying HIV-1 protein, the role of LMK1/CFL1-mediated actin dynamics in receptor function was studied. The effect of Nef expression on transcriptome was investigated following transfection of HEK293 cells with Nef-expressing plasmid. The array data identified Nef-induced inhibition of a number of genes that contain retinoid receptor binding sites in their promoters. The experiment was designed to study the effect of expression of HIV-1 Nef protein on gene expression levels in HEK293 cells. Cells were transfected in three different experiments (each time in duplicate) with Nef expressing plasmid and a plasmid that contained a non-expressing Nef construct (Nef/Stop) as control. The cells were harvested after 36 of transfection and processed for gene array.
Experiment type
transcription profiling by array 
Jun Yang <jyang@mail.nih.gov>, Mohammad Ishaq, Ven Natarajan
Investigation descriptionE-GEOD-31665.idf.txt
Sample and data relationshipE-GEOD-31665.sdrf.txt
Raw data (2)E-GEOD-31665.raw.1.zip, E-GEOD-31665.raw.2.zip
Processed data (1)E-GEOD-31665.processed.1.zip
Array designA-AFFY-143.adf.txt
R ExpressionSetE-GEOD-31665.eSet.r