E-GEOD-31642 - Expression profile in wild type, fkh2delta and fkh2-S2A mutants

Status
Released on 12 July 2012, last updated on 23 July 2012
Organism
Schizosaccharomyces pombe
Samples (6)
Array (1)
Protocols (7)
Description
Mitotic entry is accompanyed by the expression of a cluster of so called mitotic genes, whose activation is critical for mitosis in human and yeast cells. We found a link between the transcription machinery and cell cycle control network at mitosis in fission yeast, involving the Cdk8 kinase dependent phosphorylation of the fork head transcription factor Fkh2. We have generated a non-phosphorylatable fkh2 mutant (fkh2-S2A) also. We have used microarrays to study the gene expression changes in fkh2 deletion and phosphomutants vs Wt in exponentially growing fission yeast cells to detect how the phosphorylation may affect global and mitotic gene expression. RNA samples were taken from exponentially growing fission yeast cells and hybridized to affymetrix microarrays: wild type, fkh2delta and fkh2-S2A mutants
Experiment type
transcription profiling by array 
Contacts
Citation
Cyclin-dependent kinase 8 regulates mitotic commitment in fission yeast. Szilagyi Z, Banyai G, Lopez MD, McInerny CJ, Gustafsson CM. , Europe PMC 22451489
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-31642.idf.txt
Sample and data relationshipE-GEOD-31642.sdrf.txt
Raw data (1)E-GEOD-31642.raw.1.zip
Processed data (1)E-GEOD-31642.processed.1.zip
Array designA-AFFY-47.adf.txt
R ExpressionSetE-GEOD-31642.eSet.r
Links