E-GEOD-30561 - Transcription profiling by array of wild type and Alkbh1 knock out mouse embyronic stem cells

Status
Released on 1 November 2011, last updated on 19 June 2013
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (8)
Description
AlkB homolog 1 (ALKBH1) is one of nine members of the AlkB homologs in mammals. Most Alkbh1-deficient mice die during embryonic development, and survivors have severe defects in tissues originating from the ectodermal lineage. We hypothesized the phenotype to rely upon aberrant epigenetic regulation and provided evidence for ALKBH1 to be a histone H2A demethylase. We used a whole genome expression microarray to detail differentially expressed genes in embryonic stem cells lacking the Alkbh1 gene and identified distinct classes of up- and down-regulated genes during this process. Wild type and Alkbh-/- embryonic stem cells were established to identify differentially expressed genes at day 0, undifferentiated cells. We sought to obtain a subset of genes possibly explaining the observed phenotype. Total RNA was extracted and hybridized on Affymetrix GeneChips. 3 parallels were analyzed from each of the two genotypes.
Experiment types
transcription profiling by array, genetic modification design
Contacts
Elisabeth Larsen, Rune Ougland
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-30561.idf.txt
Sample and data relationshipE-GEOD-30561.sdrf.txt
Raw data (1)E-GEOD-30561.raw.1.zip
Processed data (1)E-GEOD-30561.processed.1.zip
Array designA-AFFY-45.adf.txt
Links