E-GEOD-30543 - Differential gene expression profiles between SUM149 cells transfected with control siRNA and SUM149 cells transfected with siRNA targeting tarzarotene-induced gene 1 (TIG1)

Status
Released on 3 December 2013, last updated on 2 June 2014
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (7)
Description
We identified tazarotene-induced gene 1 (TIG1) as a potential tumorigenic gene in IBC. To investigate the underlying mechanism by which TIG1 promotes tumor growth and invasiveness of IBC cells, we first sought to identify TIG1 functional partners by using DNA microarray analysis to compare gene expression profiles between SUM149 cells transfected with control siRNA and SUM149 cells transfected with siRNA targeting TIG1. We identified receptor tyrosine kinase Axl as a functional partner of TIG1. SUM149 cells transiently transfected with control siRNA or siRNA targeting TIG1 were used. Total RNA was extracted and purified using RNeasy mini kit (Qiagen, Inc.) according to the manufacturer’s instructions. The integrity of the obtained RNA was assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). The Affymetrix HGU133 plus platform was used for hybridization, staining, and imaging of the arrays by following the manufacturer’s instructions. Gene expression analysis was performed in triplicate.
Experiment type
transcription profiling by array 
Contacts
Naoto T Ueno, Xiaoping Wang
Citation
TIG1 Promotes the Development and Progression of Inflammatory Breast Cancer through Activation of Axl Kinase. Wang X, Saso H, Iwamoto T, Xia W, Gong Y, Pusztai L, Woodward WA, Reuben JM, Warner SL, Bearss DJ, Hortobagyi GN, Hung MC, Ueno NT. , Europe PMC 24014597
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-30543.idf.txt
Sample and data relationshipE-GEOD-30543.sdrf.txt
Raw data (1)E-GEOD-30543.raw.1.zip
Processed data (1)E-GEOD-30543.processed.1.zip
Array designA-AFFY-44.adf.txt
Links