E-GEOD-30440 - Toward synaptic transcriptomes: Direct sequencing and identification of RNAs actively transported by the kinesin complex from the cell body to synapses in Aplysia neurons

Status
Released on 24 April 2013, last updated on 4 May 2014
Organism
, Aplysia californica
Samples (4)
Arrays (2)
Protocols (7)
Description
Specific mRNAs are transported from the cell body to synapses where their translation can modify communication of pre-existing synapses and induce formation of new synaptic connections in response to learning. Little is known, however, about the identity of the RNAs that are actively transported and when and how these RNAs are utilized during learning. By focusing on RNAs that are associated with kinesin, a motor protein that transports gene products from the cell body to synapses, we have now applied microarrays and 454 sequencing to identify actively transported RNAs from the Aplysia central nervous system. Using a library prepared from the kinesin complex immunoprecipitated from the central nervous system (CNS), we have identified thousands of unique transcripts, of which ~600 mRNAs were annotated. Two sample comparison: kinesin IP vs. control.
Experiment type
transcription profiling by array 
Contacts
Andrea B Kohn, Eric R Kandel, Leonid L Moroz, Sathyanarayanan V Puthanveettil
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-30440.idf.txt
Sample and data relationshipE-GEOD-30440.sdrf.txt
Raw data (1)E-GEOD-30440.raw.1.zip
Processed data (1)E-GEOD-30440.processed.1.zip
Array designsA-GEOD-3635.adf.txt, A-GEOD-3636.adf.txt
Links