E-GEOD-2948 - UPRT Transgenic cells, Human Arrays

Status
Submitted on 14 July 2005, released on 16 July 2005, last updated on 18 October 2011
Organism
Homo sapiens
Samples (4)
Array (1)
Protocols (2)
Description
UPRT Transgenic and wildtype control cells of both HeLa and U373MG tissue culture lines were grown in the presence of 2mM 2,4-dithiouracil for either 6 hours (HeLa) or 8 hours (U373MG) and total RNA, not thiouracil-purified, from both transgenic and wildtype samples was compared on microarrays. An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract. Computed
Experiment type
unknown experiment type 
Contacts
Citation
Biosynthetic labeling of RNA with uracil phosphoribosyltransferase allows cell-specific microarray analysis of mRNA synthesis and decay. Cleary MD, Meiering CD, Jan E, Guymon R, Boothroyd JC.
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-2948.idf.txt
Sample and data relationshipE-GEOD-2948.sdrf.txt
Processed data (1)E-GEOD-2948.processed.1.zip
Array designA-GEOD-2631.adf.txt
Links