E-GEOD-2948 - UPRT Transgenic cells, Human Arrays
Submitted on 14 July 2005, released on 16 July 2005, last updated on 18 October 2011
UPRT Transgenic and wildtype control cells of both HeLa and U373MG tissue culture lines were grown in the presence of 2mM 2,4-dithiouracil for either 6 hours (HeLa) or 8 hours (U373MG) and total RNA, not thiouracil-purified, from both transgenic and wildtype samples was compared on microarrays. An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract. Computed
unknown experiment type
Stanford Microarray Database <email@example.com>, Michael Cleary
Biosynthetic labeling of RNA with uracil phosphoribosyltransferase allows cell-specific microarray analysis of mRNA synthesis and decay. Cleary MD, Meiering CD, Jan E, Guymon R, Boothroyd JC.