E-GEOD-2947 - Thiouracil Pulse-chase, Tachyzoites and Bradyzoites mRNA stability data

Status
Submitted on 14 July 2005, released on 16 July 2005, last updated on 18 October 2011
Organism
Toxoplasma gondii
Samples (32)
Array (1)
Protocols (2)
Description
Pulse chase measurements using thiouracil (DTU) labeling via UPRT and chasing with uracil Data from tachyzoites is labeled "DTU Pulse Chase". Two independent pulse chase experiments were performed in tachyzoites, pulse chase 1 and 2. Duplicate arrays at each timepoint were performed for pulse chase 2 (2 a and b). Data from bradyzoites are labeled "DTU Bradyzoite Pulse Chase". Two independent pulse chase experiments were performed in bradyzoites and a single set of arrays were performed for each experiment. Just one chase timepoint was used in the bradyzoite experiments, the 2 hour chase. An RNA stablity experiment design type examines stability and/or decay of RNA transcripts. User Defined
Experiment type
unknown experiment type 
Contacts
Citation
Biosynthetic labeling of RNA with uracil phosphoribosyltransferase allows cell-specific microarray analysis of mRNA synthesis and decay. Cleary MD, Meiering CD, Jan E, Guymon R, Boothroyd JC.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-2947.idf.txt
Sample and data relationshipE-GEOD-2947.sdrf.txt
Processed data (1)E-GEOD-2947.processed.1.zip
Array designA-GEOD-2632.adf.txt
Links