E-GEOD-2934 - Transcription profiling of mouse male and female blastocysts

Status
Submitted on 14 July 2005, released on 13 May 2009, last updated on 2 May 2014
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (7)
Description
Male embryos are reported to develop faster than female in the preimplantation stage. Therefore, male and female embryos can be considered phenotypically different as early as the preimplantation stage. Employing our sexing system of enhanced green fluorescent protein (EGFP) tagging X chromosomes, we compared the global gene expression pattern of male and female embryos at the blastocyst stage using DNA microarray. Experiment Overall Design: samples were collected from three independent preparations and the experiments were triplicated. Scanned microarray results were processed with Feature Extraction software (ver. 7.5, Agilent).  The hybridization experiments were duplicated in a reciprocal labeling manner to reduce dye integration bias, and total of six hybridizations were carried out for the entire analysis.  Combining plural array results and statistical analyses were carried out by Luminator software (Rosetta).
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Comparison of gene expression in male and female mouse blastocysts revealed imprinting of the X-linked gene, Rhox5/Pem, at preimplantation stages. Shin Kobayashi, Ayako Isotani, Nathan Mise, Masamichi Yamamoto, Yoshitaka Fujihara, Kazuhiro Kaseda, Tomoko Nakanishi, Masahito Ikawa, Hiroshi Hamada, Kuniya Abe, Masaru Okabe.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-2934.idf.txt
Sample and data relationshipE-GEOD-2934.sdrf.txt
Processed data (1)E-GEOD-2934.processed.1.zip
Array designA-MEXP-1183.adf.txt
Links