E-GEOD-28901 - Identification of GLI1 direct target genes in human pancreatic cancer cell line Panc-1

Status
Released on 18 July 2013, last updated on 2 June 2014
Organism
Homo sapiens
Samples (4)
Array (1)
Protocols (7)
Description
Panc-1GLI1ER and Panc-1ER were established from human pancreatic cancer cell line Panc-1. Panc-1GLI1ER cells express a chimeric transgene composed of an enhanced green fluorescent protein (EGFP)-tagged version of the amino-terminal half of GLI1, fused to the AF2 domain of the mouse estrogen receptor 1(Esr1) cDNA. On the other hand, Panc-1ER harbor only EGFP-tagged AF2 domain. To identify GLI1 direct target genes, total RNAs were purified from the cells before and 3 hours after the beta-estradiol (E2) treatment. Gene expression profiles were analyzed by AGILENT human 4x44 cDNA microarray. Keywords: Expression profiling by genome tiling array GLI1 direct target genes were identified in Panc-1 cells. Gene expression profiles of Panc-1GLI1ER and Panc-1ER cells were analyzed by AGILENT human 4x44 cDNA microarray before and 3 hours after treatment of beta-estradiol (E2) at doses of 10nM.
Experiment type
transcription profiling by array 
Contacts
Shingo Inaguma <geo@ncbi.nlm.nih.gov>, Hiroshi Ikeda, Kenji Kasai
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-28901.idf.txt
Sample and data relationshipE-GEOD-28901.sdrf.txt
Raw data (1)E-GEOD-28901.raw.1.zip
Processed data (1)E-GEOD-28901.processed.1.zip
Array designA-AGIL-28.adf.txt
Links