E-GEOD-28452 - Induction of trophoblast stem cells from embryonic stem cells by forced repression of Oct3/4

Status
Released on 17 October 2013, last updated on 24 February 2015
Organism
Mus musculus
Samples (15)
Array (1)
Protocols (7)
Description
To characterize the transdifferentiation of embryonic stem (ES) cells into trophoblast stem (TS) cells triggered by forced repression of Oct3/4, we performed whole-genome expression analysis after tetracycline (Tet)-induced knockout of Oct3/4. A Tet-inducible Oct3/4 knockout ES cell line ZHBTc4 was treated with Tet for 4 days in the presence of FGF4 and mouse embryonic fibroblasts (MEFs). A total of 15 samples from Day 0 to Day 4 were analyzed in three biological replicates.
Experiment type
transcription profiling by array 
Contacts
Itoshi NIKAIDO <itoshi.nikaido@riken.jp>, Hiroki R Ueda, Hitoshi Niwa, Itoshi Nikaido, Kenjiro Adachi
Citation
Context-Dependent Wiring of Sox2 Regulatory Networks for Self-Renewal of Embryonic and Trophoblast Stem Cells. Adachi K, Nikaido I, Ohta H, Ohtsuka S, Ura H, Kadota M, Wakayama T, Ueda HR, Niwa H. , Europe PMC 24120664
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-28452.idf.txt
Sample and data relationshipE-GEOD-28452.sdrf.txt
Raw data (2)E-GEOD-28452.raw.1.zip, E-GEOD-28452.raw.2.zip
Processed data (1)E-GEOD-28452.processed.1.zip
Array designA-AFFY-98.adf.txt
Links