E-GEOD-28451 - Transccription profiling by array of embryonic stem cell line 2TS22C and its derivative trophoblast stem cell line 22CEROH2-TSC1 with or without tetracycline-induced conditional knockout of Sox2

Status
Released on 17 October 2013, last updated on 30 April 2015
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (7)
Description
To compare the transcriptional networks governed by Sox2 in embryonic stem (ES) cells and trophoblast stem (TS) cells, we performed whole-genome expression analysis after tetracycline (Tet)-induced knockout of Sox2 in each cell type. A Tet-inducible Sox2 knockout ES cell line 2TS22C or its derivative TSC line 22CEROH2-TSC1 were treated with Tet for 4 days. A total of 12 samples from ES or TS cells cultured with or without Tet were analyzed in three biological replicates.
Experiment type
transcription profiling by array 
Contacts
Itoshi NIKAIDO <itoshi.nikaido@riken.jp>, Hiroki R Ueda, Hitoshi Niwa, Itoshi Nikaido, Kenjiro Adachi
Citation
Context-Dependent Wiring of Sox2 Regulatory Networks for Self-Renewal of Embryonic and Trophoblast Stem Cells. Adachi K, Nikaido I, Ohta H, Ohtsuka S, Ura H, Kadota M, Wakayama T, Ueda HR, Niwa H. , Europe PMC 24120664
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-28451.idf.txt
Sample and data relationshipE-GEOD-28451.sdrf.txt
Raw data (2)E-GEOD-28451.raw.1.zip, E-GEOD-28451.raw.2.zip
Processed data (1)E-GEOD-28451.processed.1.zip
Array designA-AFFY-98.adf.txt
Links