E-GEOD-28451 - Conditional knockout of Sox2 in embryonic and trophoblast stem cells.

Status
Released on 17 October 2013, last updated on 14 November 2013
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (7)
Description
To compare the transcriptional networks governed by Sox2 in embryonic stem (ES) cells and trophoblast stem (TS) cells, we performed whole-genome expression analysis after tetracycline (Tet)-induced knockout of Sox2 in each cell type. A Tet-inducible Sox2 knockout ES cell line 2TS22C or its derivative TSC line 22CEROH2-TSC1 were treated with Tet for 4 days. A total of 12 samples from ES or TS cells cultured with or without Tet were analyzed in three biological replicates.
Experiment type
transcription profiling by array 
Contacts
Itoshi NIKAIDO <itoshi.nikaido@riken.jp>, Hiroki R Ueda, Hitoshi Niwa, Itoshi Nikaido, Kenjiro Adachi
Citation
Context-dependent wiring of sox2 regulatory networks for self-renewal of embryonic and trophoblast stem cells. Adachi K, Nikaido I, Ohta H, Ohtsuka S, Ura H, Kadota M, Wakayama T, Ueda HR, Niwa H. , Europe PMC 24120664
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-28451.idf.txt
Sample and data relationshipE-GEOD-28451.sdrf.txt
Raw data (2)E-GEOD-28451.raw.1.zip, E-GEOD-28451.raw.2.zip
Processed data (1)E-GEOD-28451.processed.1.zip
Array designA-AFFY-98.adf.txt
Links