E-GEOD-27855 - Genome wide expression analysis of EST-induced EBE-XVE using Affymetrix ATH1 array

Status
Released on 30 March 2013, last updated on 2 June 2014
Organism
Arabidopsis thaliana
Samples (6)
Array (1)
Protocols (8)
Description
Global transcriptome patterns were determined in XVE-14 and wild-type seedlings induced for 45 min b-estradiol in order to identify the genes early regulated by EBE transcription factor. We used microarrays to identify genes differentially expressed in EST-inducible EBE over-expression line #14 compared to wild-type plants, 45 min after 2µM EST induction. Three independent biological replications were performed. In order to identify potential direct/early target genes of EBE transcription factor, estradiol inducible overexpression system was used. Three week old Arabidopsis EBE-XVE (line 14) and WT seedlings were treated with ß-estradiol (2µM) for 45 min. RNA was isolated from shoot and subjected to hybridization on Affymetrix microarrays. Experiment was performed in 3 biological replications and genes differentially expressed between estradiol treated EBE-XVE and WT plants were identified as potential early targets of EBE.
Experiment type
transcription profiling by array 
Contact
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-27855.idf.txt
Sample and data relationshipE-GEOD-27855.sdrf.txt
Raw data (1)E-GEOD-27855.raw.1.zip
Processed data (1)E-GEOD-27855.processed.1.zip
Array designA-AFFY-2.adf.txt
Links