E-GEOD-27238 - FACS-array profiling in retinal endothelial cells from living mouse retinas

Status
Released on 17 April 2011, last updated on 26 April 2011
Organism
Mus musculus
Samples (18)
Arrays (3)
Protocols (7)
Description
Deregulated retinal angiogenesis directly cause vision loss in many ocular diseases, such as diabetic retinopathy and retinopathy of prematurity. To identify endothelial-specific genes expressed in angiogenic retinal vessels, we purified genetically labeled endothelial cells from Tie2-GFP transgenic mice and performed gene expression profiling using DNA microarray. To find out genes associated with angiogenesis, comparisons of microarray data were carried out between GFP-negative non-endothelial retinal cells and GFP-positive retinal endothelial cells in angiogenic P8 retina. Eighteen arrays are included. Utilizing fluorescence-activated cell sorting (FACS), we isolated endothelial cells as GFP-positive cells from P8 retina in homozygous Tie2-GFP transgenic mice. GFP-negative cells were served as non-endothelial control. RNA extracts from sorted cells were amplified and then hybridized to Affymetrix MGU74v2 series arrays in triplicate. 
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-27238.idf.txt
Sample and data relationshipE-GEOD-27238.sdrf.txt
Raw data (1)E-GEOD-27238.raw.1.zip
Processed data (1)E-GEOD-27238.processed.1.zip
Array designsA-AFFY-6.adf.txt, A-AFFY-7.adf.txt, A-AFFY-8.adf.txt
R ExpressionSetE-GEOD-27238.eSet.r
Links