E-GEOD-27212 - Gene expression in spinal cord neurons grown on polyornithine or on carbon nanotube (CNT)-based substrates

Status
Released on 5 August 2013, last updated on 2 June 2014
Organism
Rattus norvegicus
Samples (6)
Array (1)
Protocols (7)
Description
Carbon nanotubes are cylindrically-shaped carbon nanostructures, made up of layers of graphene rolled onto themselves, with diameters similar to those of neuronal processes. In the last decade, CNT have been used as biocompatible growing substrates for neuronal attachment, differentiation and growth. In the perspective of new developments in tissue engineering, and in particular in spinal cord repair strategies, based on the use of CNTs, our aim is to clarify the biophysical interactions between CNTs and spinal cord neurons, studying the development of the morphological and functional characteristics of spinal neurons grown on CNT-based interfaces. In this context, we used microarrays to show whether cultured dissociated spinal neurons show a difference in gene expression when grown in control conditions (polyornithine-covered substrates) or on CNT-based substrates. Gene expression profiling was evaluated from 3 replicates each of neurons grown on nanotubes and control conditions.
Experiment type
transcription profiling by array 
Contacts
Paola Roncaglia <geo@ncbi.nlm.nih.gov>, Alessandra Fabbro, Antonella Sucapane, Claudia Carrieri, Laura Ballerini, Stefano Gustincich
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-27212.idf.txt
Sample and data relationshipE-GEOD-27212.sdrf.txt
Raw data (1)E-GEOD-27212.raw.1.zip
Processed data (1)E-GEOD-27212.processed.1.zip
Array designA-AFFY-43.adf.txt
Links