E-GEOD-26524 - Expression data from differentiating Flk1- and Flk1+ ES cells expressing Snail during Wnt inhibition

Status
Released on 23 October 2013, last updated on 2 June 2014
Organism
Mus musculus
Samples (3)
Array (1)
Protocols (7)
Description
ES cells differentiated in the presence of the Wnt inhibitor DKK1 fail to express the transcription factor Snail and undergo EMT or mesoderm differentiation. We generated an ES cell line, A2.snail, that induced Snail expression upon addition of doxycycline addition. Microarrays were used to gain a global picture of Flk1- and Flk1+ cells generated one day after Snail was expressed during Wnt inhibition. A2.snail ES cells, which express Snail upon addition of doxycycline, were differentiated as embryoid bodies in differentiation media and DKK1. Snail-induced cultures uniquely develop a select population of Flk1+ cells. Total RNA was harvested from sorted control (no doxycycline) Flk1- cells and sorted Snail-induced (doxycycline at day 2) Flk1- and Flk1+ cells at day 3 of differentiation.
Experiment type
transcription profiling by array 
Contacts
Jennifer Gill <geo@ncbi.nlm.nih.gov>, Ellen M Langer, Jennifer G Gill, Kenneth M Murphy, Mi Cai, Michael Kyba, Robert C Lindsley, Theresa L Murphy
Citation
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-26524.idf.txt
Sample and data relationshipE-GEOD-26524.sdrf.txt
Raw data (1)E-GEOD-26524.raw.1.zip
Processed data (1)E-GEOD-26524.processed.1.zip
Array designA-AFFY-45.adf.txt
Links