E-GEOD-26524 - Expression data from differentiating Flk1- and Flk1+ ES cells expressing Snail during Wnt inhibition
Released on 23 October 2013, last updated on 2 June 2014
ES cells differentiated in the presence of the Wnt inhibitor DKK1 fail to express the transcription factor Snail and undergo EMT or mesoderm differentiation. We generated an ES cell line, A2.snail, that induced Snail expression upon addition of doxycycline addition. Microarrays were used to gain a global picture of Flk1- and Flk1+ cells generated one day after Snail was expressed during Wnt inhibition. A2.snail ES cells, which express Snail upon addition of doxycycline, were differentiated as embryoid bodies in differentiation media and DKK1. Snail-induced cultures uniquely develop a select population of Flk1+ cells. Total RNA was harvested from sorted control (no doxycycline) Flk1- cells and sorted Snail-induced (doxycycline at day 2) Flk1- and Flk1+ cells at day 3 of differentiation.
transcription profiling by array
Jennifer Gill <firstname.lastname@example.org>, Ellen M Langer, Jennifer G Gill, Kenneth M Murphy, Mi Cai, Michael Kyba, Robert C Lindsley, Theresa L Murphy
Snail promotes the cell-autonomous generation of Flk1(+) endothelial cells through the repression of the microRNA-200 family. Gill JG, Langer EM, Lindsley RC, Cai M, Murphy TL, Murphy KM. , Europe PMC 21861700