E-GEOD-25549 - Polycomb Protein Ezh1 Promotes RNA Polymerase II Elongation

Status
Released on 23 November 2011, last updated on 3 May 2014
Organism
Mus musculus
Samples (30)
Protocols (8)
Description
Polycomb group (PcG) proteins initiate the formation of repressed chromatin domains and regulate developmental gene expression. A mammalian PcG protein, Enhancer of Zeste homolog 2 (Ezh2), triggers transcriptional repression by catalyzing the addition of methyl groups onto lysine-27 of histone H3 (H3K27me2/3)1. This action facilitates the binding of other PcG proteins to histone H3 and compaction of chromatin. Interestingly, there exists a paralog of Ezh2, termed Ezh1, whose primary function remains unclear. Here, we provide evidence for genome-wide association of Ezh1 with active epigenetic marks, RNA polymerase II (PolII) and mRNA production. Ezh1 depletion reduced global PolII occupancy within gene bodies and resulted in delayed transcriptional activation during differentiation of skeletal muscle cells. Conversely, ectopic expression of wild-type Ezh1 led to premature gene activation and rescued PolII-elongation defects in Ezh1-depleted cells. Collectively, these findings reveal an unanticipated role of a PcG protein in promoting mRNA transcription. Examination of 3 different histone modifications, 3 modified forms of RNA polymerase II, Ezh1, Ezh2 and mRNA levels in a skeletal muscle cells at various developmental stages.
Experiment types
ChIP-seq, RNA-seq of coding RNA 
Contacts
Kambiz Mousavi <mousavik@mail.nih.gov>, A H Wang, Hossein Zare, Vittorio Sartorelli
Citation
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-25549.idf.txt
Sample and data relationshipE-GEOD-25549.sdrf.txt
Processed data (27)Click to browse processed data
Links